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Fermentation / Cell Culture > Sterile Sampling Probes > Operation and Applications Examples
 
 
 
 
 
 
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Operation and Application Examples

The FISP® is inserted into a side or top port of the vessel. it is immersed into the fermentation broth of culture media and sterilized in the vessel. A peristaltic pump (or the vessel's own hydrostatic pressure) withdraws a sample through the tubular membrane and into the probe's stainless steel network of grooves. the sample flows through the network of grooves into a central channel where the cell-free sample can be directed to an on-line analyser, fraction collector or sample container. Fluid flow rate is dependant on the viscosity of the media and will not exceed 2ml/min.

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Applications examples
Organisms
fermentation Period (hr)
Flowrate (ml/min)
Bacteria
E. coli K12
E. coli Recomb
Strepto coccus Pheu
de 24 à 72
40
9
0,75
1,5
1,0
Yeast
Sacch. cerevisiae
Sacch. cerevisiae
48
de 24 à 150
1,0
0,80
Fungal
Acremonium chrysogenum
Sodoptera frugi
160
200
1,0
0,80
Cell Culture
BHK 21, CHO
NSO
42 (jours)
37 (jours)
0,50
0,75

Presentation

Benefits

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